FastKing Ib Kauj Ruam RT-qPCR cov khoom siv

Ib kauj ruam Real-Time RT-PCR nrog SYBR-Green.

FastKing Ib Kauj Ruam RT-qPCR Cov Khoom Siv tau tsim los rau Lub Sijhawm Ib Leeg Ib Qho RT-qPCR los ntawm kev siv SYBR Green I. Cov khoom siv tuaj yeem siv hauv lub sijhawm tiag tiag ib kauj ruam RT-qPCR ntawm RNA lub hom phiaj, nrog rov qab sau thiab PCR ua qhov sib ntxiv hauv tib lub raj. Yog li ntawd txoj haujlwm yog yooj yim, thiab nws txo qis kev pheej hmoo ntawm kev kis kab mob.

25 × RT-PCR Enzyme Mix hauv cov khoom siv no yog qhov ua ntej ntawm TIANGEN cov ntawv tshiab thim rov qab transcriptase (FastKing RTase), cov tshuaj tiv thaiv kab mob tshiab hloov kho sai Taq DNA polymerase thiab RNase Inhibitor. FastKing RTase yog hom tshiab ntawm kev hloov pauv hloov transcriptase. Nrog rau qhov tshwj xeeb ntxiv hydrophobic cov qauv, uas muaj zog RNA kev sib raug zoo thiab thermal stability. FastKing RTase tau txhim kho kev thim rov qab cov txiaj ntsig tau zoo thiab muaj peev xwm txuas ntxiv RNA tus qauv nrog cov qauv nyuaj thib ob. Hom tshiab ntawm hotstartTaq DNA polymerase nrog kev ua tau zoo tau siv hauv txheej txheem PCR, ua rau PCR cov tshuaj tiv thaiv tom qab cov ntawv sau rov qab muaj qhov ua tau zoo dua qub thiab tshwj xeeb. Ib qho ntxiv, 2 × FastKing RT-qPCR Buffer (SYBR Green) hauv cov khoom no yog cov txheej txheem txheej txheem tshwj xeeb uas tau ua kom zoo tshaj rau ob qho tseem ceeb enzymes, suav nrog cov khoom tsim nyog ionic, dNTPs, SYBR Green zas, ib kauj ruam ruaj khov thiab txhim kho, tuaj yeem ntseeg tau tias FastKing RTase thiab qhov pib tshiabTaq DNA polymerase tuaj yeem ua lub luag haujlwm zoo tshaj plaws hauv tag nrho cov txheej txheem txheej txheem ib kauj ruam.

Cov khoom siv no muab cov qauv nkhaus zoo hauv ntau qhov chaw ntawm qhov muaj nuj nqis. Nws tuaj yeem txheeb xyuas qhov tseeb thiab ntau qhov ntau ntawm cov hom phiaj siab thiab qis nrog cov noob zoo nrog kev rov ua tau zoo thiab muaj kev ntseeg siab.