HRM Analysis Kit (EvaGreen)

Nta

Resolution Kev daws teeb meem siab: Txais siv EvaGreen cov xim pleev xim, uas muaj kev daws teeb meem siab ntawm cov nkhaus yaj hauv lub xeev khov thiab tuaj yeem paub qhov txawv ntawm ib puag ncig.
Specific Qhov tshwj xeeb siab: Siv cov tshuaj tiv thaiv kab mob hloov kho qhov kub DNA DNA polymerase kom txo qis qhov tshwj xeeb nthuav dav thiab txhim kho qhov tshwj xeeb.
Stability Kev nyab xeeb siab: Ua tib zoo kho qhov tsis zoo ua rau kom ruaj khov ntawm cov nkhaus yaj thiab txhim kho kev ntseeg tau ntawm cov txiaj ntsig.
■ ROX kho: ROX zas xim tau ntim cais, uas hloov pauv tau kom siv tau thiab muaj txiaj ntsig zoo dua.

Kev qhia tshwj xeeb

Hom: Antibody hloov pauv Taq DNA polymerase, EvaGreen.
Cov ntawv thov: Paub SNP ntaus ntawv; Tsis paub tshuaj ntsuam xyuas SNP; Tsis paub cov noob mutant scanning; Methylation PCR tsom xam.

Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)

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Genomic DNA tau muab rho tawm los ntawm 100 μl ntawm tib neeg cov ntshav coj los siv TIANamp Blood DNA Kit. 50 ng DNA tau thauj khoom rau lub sijhawm kuaj PCR tiag. Raws li NCBI SNP lub tsev qiv ntawv, paub SNP ntawm FEN1 noob (RS 174538) raug xaiv thiab kuaj pom siv Roche LightCycle480. Cov txiaj ntsig yog raws li hauv qab no:

Cov txiaj ntsig tau qhia tias HRM Kev Txheeb Xyuas Khoom yog qhov zoo tshaj plaws SNP qhov chaw tshawb xyuas txoj hauv kev nrog rov ua dua ntawm cov nkhaus yaj ntawm tib lub genotype, kev daws teeb meem meej ntawm cov genotypes sib txawv thiab tsis muaj kev txiav txim siab tsis raug.

SNP cov ntaub ntawv: …… CGGAAGAACACGTCG [A/G] CAGGAGCAGGCGCCT …… (Allele Zaus: A = 0.314, G = 0.686)

Primer: Rau 108 bp fragment (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)