Magnetic Blood Genomic DNA Kit Featured Image

Cov Khoom Sib Nqus Ntshav Genomic DNA

Ua kom muaj txiaj ntsig zoo ntawm kev ua kom muaj txiaj ntsig zoo DNA ntawm 100 μl-1 ml ntshav.

Cov khoom siv tau txais cov hlaws dai sib nqus nrog qhov sib cais ua haujlwm sib txawv thiab tsis muaj qhov tshwj xeeb los cais thiab ua kom lub cev muaj DNA zoo los ntawm 100 μl-1 ml ntawm cov ntshav. Cov cim sib nqus sib nqus hlaws dai muaj kev sib raug zoo rau nucleic acid hauv qee qhov xwm txheej. Thaum cov xwm txheej hloov pauv, cov hlaws dai sib nqus tso tawm cov adsorbed nucleic acid, yog li ua tiav lub hom phiaj ntawm kev sib cais sai thiab ua kom huv ntawm nucleic acid. Tag nrho cov txheej txheem muaj kev nyab xeeb thiab yooj yim. Nrog rau qhov muab rho tawm ntawm cov DNA DNA tawg, ua kom siab dawb huv thiab txhim khu kev qha, nws yog qhov tshwj xeeb tshaj yog tsim rau kev rho tawm ntawm cov chaw ua haujlwm siab. DNA tshem tawm los ntawm cov khoom siv tuaj yeem tsim nyog rau ntau yam kev ua haujlwm yooj yim, suav nrog kev zom zaub mov, PCR, lub sijhawm PCR tiag tiag, kev tsim tsev qiv ntawv, Yav qab teb blot, kuaj pom nti, qhov ua tau zoo dhau los thiab lwm yam kev sim.

Miv. Tsis yog	Ntim Loj
4992402	50 ua prep
4992403	200 kev npaj ua ntej
4992976	1000 kev npaj

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Khoom nthuav dav

Kev sim ua piv txwv

FAQ

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Nta

■ Yooj yim thiab nrawm: Ultra-pure genomic DNA tuaj yeem tau txais hauv 1 teev.
Through Kev Tshaj Tawm Siab: Nws tuaj yeem sib sau ua ke cov cuab yeej siv ntawm pipetting thiab pas nrig sib nqus kom ua tiav qhov siab dhau los ntawm kev sim ua kom rho tawm.
■ Muaj kev nyab xeeb thiab tsis muaj kuab lom: Tsis xav tau cov tshuaj reagents organic xws li phenol/ chloroform.
Pur Siab dawb huv: Cov DNA tau txais muaj lub siab dawb huv thiab tuaj yeem siv ncaj qha rau hauv kev txheeb xyuas cov nti, ua kom dhau qib sib xws thiab lwm yam kev sim.

Nyem rho tawm

Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)

Yav dhau los: TIANamp Cov quav DNA DNA

Tom ntej: TIANquick FFPE DNA Kit

DNA NanoDrop ntsuas cov txiaj ntsig ntawm DNA rho tawm los ntawm 200 bloodl ntshav siv TIANGEN Magnetic Blood Genomic DNA Kit thiab cov khoom muaj feem cuam tshuam los ntawm Tus Muag Khoom G

Siv TIANGEN Magnetic Blood Genomic DNA Kit thiab cov khoom muaj feem cuam tshuam los ntawm Tus Muab Khoom G txhawm rau rho tawm DNA los ntawm 200 μl ntshav. DNA tau suav nrog 200 μl Tsis TB. Siv 5 μl rau electrophoresis, thiab 2 μl rau NanoDrop 2000 txhawm rau ntsuas kev mloog zoo thiab huv. Cov txiaj ntsig tau qhia tias cov txiaj ntsig rho tawm ntawm TIANGEN Magnetic Blood Genomic DNA Kit yog 18% siab dua li ntawm Tus Neeg Muag Khoom G.
M: TIANGEN Marker D15000

Q: Me me lossis tsis muaj DNA hauv qhov tsim nyog.

A-1 Tsawg concentration ntawm cov cell lossis tus kab mob hauv qhov pib ua piv txwv -Ua kom muaj kev txaus siab ntawm cov cell lossis kab mob.

A-2 Tsis txaus lysis ntawm cov qauv-Cov qauv tsis tau muab sib xyaw kom huv nrog cov lysis tsis. Nws tau hais kom ua kom sib tov sib xyaw los ntawm mem tes-vortexing rau 1-2 zaug. -Tsis txaus lysis ntawm tes ua los ntawm kev ua haujlwm qis ntawm proteinase K. - Tsis txaus cell lysis lossis protein degradation vim lub sijhawm da dej sov tsis txaus. Nws tau hais kom txiav cov ntaub so ntswg rau hauv daim me me thiab ncua sijhawm da dej kom tshem tag nrho cov seem hauv lysate.

A-3 Tsis txaus DNA adsorption. -Tsis muaj ethanol lossis qhov feem pua tsawg dua 100% ethanol tau ntxiv ua ntej lysate tau hloov mus rau kab kem.

A-4 Tus nqi pH ntawm kev tshem tawm qhov tsis zoo heev. -Kho cov pH kom nyob nruab nrab ntawm 8.0-8.3.

Q: DNA tsis ua tau zoo hauv kev sim tshuaj enzymatic nqes hav.

Cov seem ethanol hauv cov eluent.

-Txhua yam tseem tshuav ntxuav tsis tau PW hauv qhov tsim nyog. Ethanol tuaj yeem raug tshem tawm los ntawm kev ua haujlwm centrifuging rau 3-5 feeb, thiab tom qab ntawd muab tso rau ntawm chav sov lossis 50 ℃ qhov cub rau 1-2 feeb.

Q: DNA puas tsuaj

A-1 Cov qauv tsis tshiab. - Txheeb tawm qhov piv txwv DNA zoo raws li kev tswj hwm txhawm rau txiav txim siab tias DNA hauv cov qauv tau puas lawm.

A-2 Kev kho ua ntej tsis raug. -Caused los ntawm cov kua nitrogen sib tsoo ntau dhau, rov ua kom muaj dej noo, lossis ntau dhau ntawm cov qauv.

Q: Yuav ua li cas thiaj ua tau qhov pretreatment rau gDNA rho tawm?

Kev kho ua ntej yuav tsum sib txawv rau cov qauv sib txawv. Rau kev kuaj cov nroj tsuag, nco ntsoov ua tib zoo zom hauv cov kua nitrogen. Rau kev kuaj tsiaj, homogenate lossis ua tib zoo zom hauv cov kua nitrogen. Txog kev kuaj nrog cov phab ntsa ntawm tes uas nyuaj rau tawg, xws li G+ cov kab mob thiab cov poov xab, nws tau hais kom siv lysozyme, lyticase lossis cov txheej txheem kho tshuab txhawm rau txhawm rau phab ntsa ntawm tes.

Q: Dab tsi yog qhov sib txawv ntawm peb tsob nroj gDNA cov khoom siv rho tawm 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit tau txais cov txheej txheem raws kab uas xav tau chloroform rau kev rho tawm. Nws yog qhov tshwj xeeb tshaj yog haum rau ntau yam piv txwv cog, ntxiv rau cog cov hmoov qhuav. Hi-DNAsecure Plant Kit tseem yog kem-raws, tab sis tsis xav tau phenol/chloroform rho tawm, ua rau nws nyab xeeb thiab tsis muaj tshuaj lom. Nws yog qhov tsim nyog rau cov nroj tsuag uas muaj polysaccharides siab thiab cov ntsiab lus polyphenol. 4992709/4992710 DNAquick Plant System tau txais cov txheej txheem ua kua. Phenol/chloroform rho tawm tsis xav tau ib yam nkaus. Cov txheej txheem kev ua kom huv yog yooj yim thiab nrawm nrog tsis muaj kev txwv rau qhov pib ua piv txwv, yog li cov neeg siv tuaj yeem hloov kho qhov yoog raws qhov xav tau ntawm kev sim. Loj me me ntawm gDNA cov khoom tawg tuaj yeem tau txais nrog cov txiaj ntsig siab.

Dab tsi yog qhov kwv yees kwv yees ntawm gDNA los ntawm 1 ml ntshav coj los ntawm TIANamp Cov Ntshav DNA Cov Khoom?

Lub genomic DNA tau muab rho tawm los ntawm ntau qhov sib txawv ntawm tib neeg cov ntshav tag nrho los ntawm TIANamp Blood DNA Kit. Cov txiaj ntsig yog raws li hauv qab no. Cov txiaj ntsig tau teev tseg raws li siv nkaus xwb, qhov txiaj ntsig rho tawm tiag tiag nyob ntawm qhov xwm txheej ntawm kev kuaj.

Q: Puas yog 4992207/4992208 thiab 4992722/4992723 tuaj yeem siv los tshem tawm cov ntshav txhaws DNA?

Cov ntshav khov DNA rho tawm tuaj yeem ua tiav siv cov tshuaj reagents muab hauv ob qho khoom siv no los ntawm kev hloov pauv cov txheej txheem tshwj xeeb rau kev qhia tshwj xeeb rau cov ntshav khov DNA tshem tawm. Daim ntawv mos ntawm cov ntshav khov DNA cov txheej txheem rho tawm tuaj yeem muab tawm thaum thov.

Q: Thaum thov TIANamp Genomic DNA Kit, yuav ua li cas txhawm rau cov ntaub so ntswg tshiab rau hauv kev tshem tawm ntawm tes?

Nthuav cov qauv tshiab nrog 1 ml PBS, cov kua ntsev ib txwm lossis TE tsis. Ua kom tiav cov homogenize cov qauv los ntawm homogenizer thiab sau cov nag mus rau hauv qab ntawm lub raj los ntawm kev ua centrifuging. Muab cov supernatant tso tseg, thiab rov ua kom cov nag los nrog 200 μl tsis GA. Kev ua kom huv DNA hauv qab no tuaj yeem ua raws li cov lus qhia.

Q: Yuav ua li cas xaiv cov khoom lag luam rau kev tshem tawm DNA los ntawm ntshav, ntshav thiab kuaj lub cev?

Txog kev ua kom huv ntawm gDNA hauv ntshav, ntshav thiab kuaj lub cev, TIANamp Micro DNA Kit tau pom zoo. Txog kev ua kom huv ntawm tus kab mob gDNA los ntawm kev kuaj ntshav/ntshav, TIANamp Virus DNA/RNA Kit tau pom zoo. Txog kev ua kom huv ntawm cov kab mob gDNA los ntawm cov ntshav thiab cov ntshav ntshav, TIANamp Cov Kab Mob Cov Kab Mob DNA tau pom zoo (lysozyme yuav tsum suav nrog rau cov kab mob zoo). Txog cov hnoos qeev, Hi-Swab DNA Kit thiab TIANamp Bacteria DNA Kit tau pom zoo.

Q: Yuav ua li cas xaiv cov khoom siv rau gDNA rho tawm los ntawm kev kuaj cov pwm?

DNAsecure Plant Kit lossis DNAquick Plant System tau pom zoo rau kev tshem tawm cov noob genome. Txog cov poov xab genome rho tawm, TIANamp Poov Cov Khoom DNA tau pom zoo (lyticase yuav tsum tau npaj tus kheej).

Sau koj cov lus ntawm no thiab xa tuaj rau peb