Thaum lub Kaum Ob Hlis 2019, cov xwm txheej mob ntsws tsis paub txog tau pib los ntawm Wuhan, Xeev Hubei, thiab sai sai kis mus rau ntau lub xeev thiab nroog hauv Suav teb, thiab ntau lwm lub tebchaws thaum Lub Ib Hlis 2020. Raws li 22:00 teev tsaus ntuj Lub Ib Hlis 27, 28 tus neeg uas tau lees paub thiab 5794 xav tias yog 2019-nCov kis tau tshaj tawm hauv Suav teb. Covqhov chaw kis tus kab mob tau suav tias yog Rhinolophus, thiab cov neeg tuag tam sim no yog 2.9%.
Thaum Lub Ib Hlis 12, 2020, Lub Koom Haum Saib Xyuas Kev Noj Qab Haus Huv Ntiaj Teb (WHO) tshaj tawm tias kev mob ntsws hnoos qeev tau tshwm sim los ntawm hom kab mob tshiab (2019-nCov). Coronavirus yog hom kab mob uas kis rau tsiaj. Hom kab mob nucleic acid yog ib leeg RNA. Nyob rau tib lub sijhawm, WHO tseem tau tshaj tawm cov ntaub ntawv nucleic acid sib qhia ntawm nCov qhia los ntawm cov kws tshawb fawb Suav, uas tau muab lub hauv paus kev tshawb pom molecular rau kev kuaj pom tus kab mob thiab tau ua kom pom sai thiab txheeb xyuas cov kab mob tau.
WHO qhia tias cov tib neeg uas muaj kab mob ua pa hnyav xws li hnoos, ua npaws, ua pa nyuaj rau lub ntsws thiab tau mus rau Wuhan tsis pub dhau 14 hnub lossis tau ntsib kev sib cuag nrog lwm tus neeg mob yuav tsum raug tshuaj xyuas kom zoo. Thaum Lub Ib Hlis 17, 2020, WHO tau tshaj tawm "Kev kuaj sim rau 2019 tshiab tus kabmob coronavirus (2019-nCoV) hauv kev xav tias tib neeg kis tus kabmob, Qhia ib ntus, 17 Lub Ib Hlis 2020". Cov lus taw qhia taw qhia tias cov hnoos qeev los khaws los ntawm cov neeg mob muaj xws li cov pa ua pa (nasopharynx thiab oropharyngeal swabs, hnoos qeev, hnoos hnoos hnoos qeev, thiab lwm yam) thiab cov hnoos qeev, raws li hauv qab no:
*Rau kev thauj cov qauv mus kuaj tus kab mob, siv VTM (kis thauj nruab nrab) uas muaj cov tshuaj tua kab mob thiab tshuaj tua kab mob ntxiv. Txog kab mob los yog kab mob fungal, thauj kom qhuav lossis hauv qhov me me ntawm cov dej tsis huv. Tsis txhob rov khov dua thiab ua kom yaj ntawm cov hnoos qeev.
Ib cag los ntawm cov ntaub ntawv sau tshwj xeeb uas tau hais hauv lub rooj kuj tseem muaj lwm cov ntaub ntawv thiab khoom siv muaj xws li: thauj cov ntim thiab ntim cov hnab ntim khoom ntim thiab ntim khoom, txias thiab ntim txias lossis cov dej khov qhuav, cov khoom siv tsis muaj ntshav (piv txwv li koob, koob txhaj tshuaj thiab raj), cov ntawv cim thiab cov cim ruaj khov, PPE, cov khoom siv rau kev ua kom huv ntawm qhov chaw.
Txog kev kuaj pom tus kab mob, cov hnoos qeev tuaj yeem siv los kuaj tus kab mob los ntawm txoj hauv kev tiv thaiv kab mob, thaum RTqPCR tau pom zoo kom txheeb xyuas nCov nucleic acid kom nrawm dua thiab pom tseeb dua. Raws li tus kab mob
ib theem zuj zus tau paub, cov ntsiab lus ntawm viral nucleic acid hauv cov hnoos qeev tuaj yeem kuaj pom sai sai los ntawm
xaiv cov tshuaj reagents uas tsim nyog thiab sib piv cov primers
(Kev kuaj pom tus kab mob Wuhan coronavirus 2019 los ntawm lub sijhawm tiag tiag RTPCR, Cov txheej txheem thiab kev ntsuas ua ntej thaum Lub Ib Hlis 13, 2020) Ua tus thawj coj hauv thaj tsam ntawm nucleic acid rho tawm thiab kuaj pom hauv Suav teb, TIANGEN BIOTECH (BEIJING) CO., LTD. tau muab tus kab mob tes-taw-qhov ncauj thiab tus kab mob khaub thuas A (H1N1) kuaj pom cov tshuaj reagents rau ntau dua 10 lab tus tib neeg. Xyoo 2019, TIANGEN tus kab mob nucleic acid rho tawm thiab tshawb pom cov tshuaj reagents tau siv rau 30 lab tus neeg African cov npua npua piv txwv, ua rau muaj txiaj ntsig zoo rau kev kuaj mob thiab tiv thaiv African classical swine fever hauv Suav teb. TIANGEN tsis tsuas yog muab cov kab mob tshem tawm sai thiab raug thiab tshawb pom cov tshuaj reagents, tab sis kuj tseem muab cov cuab yeej sib tsoo yooj yim thiab muaj txiaj ntsig thiab tsis siv neeg nucleic acid extractors, uas yog ua tiav cov txheej txheem daws teeb meem rau kev rho tawm thiab kuaj pom.
Automated Nucleic Acid Extraction Solution
TIANGEN Automated Nucleic Acid Extractors yog lub tshuab tsis siv neeg rau nucleic acid rho tawm siv txoj siv sib nqus hlaws. Daim ntawv thov ntawm cov platform no tsis tsuas yog txo qis kev ua haujlwm ntawm kev tshuaj xyuas thiab cais cov tuam tsev, tab sis tseem txo qis kev ua haujlwm tsis raug ntawm phau ntawv rho tawm, thiab ua kom muaj kev sib xws thiab ruaj khov ntawm qhov zoo ntawm cov kua nucleic acid.
TIANGEN Automated Nucleic Acid Extractor muaj ntau yam kev nkag mus (suav nrog 16, 24, 32, 48, 96 raws), thiab cov tshuaj sib txuam sib xyaw tuaj yeem siv rau nucleic acid rho tawm los ntawm ntau hom qauv. TIANGEN tseem muab kev txhim kho cov tshuaj reagent thiab cov cuab yeej siv ua ke raws li kev sim sib txawv uas xav tau.
TGrinder H24 Cov Ntawv Homogenizer
● Siv dav rau kev sib tsoo thiab homogenizing ntau cov ntaub so ntswg thiab quav quav
● Nrog kev sib tsoo homogenization quab yuam 2-5 npaug ntawm cov cuab yeej ib txwm muaj
● Uniform sib tsoo thiab homogenization, zam kev hla kev sib kis
● Cov cuab yeej tiv thaiv tsis siv neeg los tiv thaiv kev nyab xeeb ntawm cov neeg ua haujlwm kuaj ntshav
TGuide S32 Automated Nucleic Acid Extractor
● Qauv piv txwv: 1-32 kuaj
Volume Ua ntim: 20-1000 μl
Type Piv txwv hom: Ntshav, hlwb, nqaij, quav, kab mob thiab lwm yam piv txwv
Time Ua lub sijhawm: Mus txog 8 min kom tau txais cov kab mob nucleic acid
● Tswj hom: Dual tswj hom ntawm Windows ncoo thiab khawm lub vijtsam
Development Kev txhim kho Platform: Qhib platform, pub dawb kom phim cov tshuaj reagents
● TGuide S32 Sib Nqus Viral DNA/RNA Cov Khoom (DP604)
Application Daim ntawv thov dav: Tus kab mob zoo DNA/RNA tuaj yeem lim tau los ntawm cov ntshav, ntshav, cov hnoos qeev, cov ntaub so ntswg kho thiab ntau yam tshuaj tiv thaiv kab mob.
● Yooj yim thiab muaj txiaj ntsig: Cov khoom no tau ua tiav nrog TGuide S32 Automated Nucleic Acid Extractor, uas tuaj yeem tshem tawm cov kab mob DNA/RNA nrog cov txiaj ntsig siab, siab dawb huv, ruaj khov thiab ntseeg tau zoo.
Applications Daim ntawv thov nqes hav: Cov kua qaub nucleic uas tsim nyog yog tsim rau kev sim hauv qab ntawm tus kab mob PCR thiab PCR lub sijhawm tiag.
Phau ntawv Nucleic Acid Extraction Solution
Raws li tus thawj coj hauv thaj tsam ntawm nucleic acid rho tawm thiab nrhiav pom, TIANGEN muaj cov txheej txheem tshaj plaws ntawm cov khoom siv nucleic acid rho tawm hauv Suav teb, uas yog qhov zoo tshaj rau kev kuaj pom tus kabmob coronavirus los ntawm txhua yam kev kuaj: ntshav, ntshav/ntshav, nqaij, swab, tus kabmob , lwm yam
TIANamp Cov Kab Mob DNA/RNA Cov Khoom (DP315)
Efficiency Ua haujlwm tau zoo: Cov kab mob zoo DNA thiab RNA tuaj yeem tau los ntawm kev ua kom huv sai nrog rov ua dua.
Pur Siab dawb huv: Ua kom tiav tshem tawm cov pa phem thiab cov tshuaj tiv thaiv rau hauv qab daim ntawv thov.
Safety Muaj kev nyab xeeb siab: Tsis siv cov organic reagent los yog nag lossis daus nag lossis daus tsis xav tau.
RNA Virus Detection Solution
1. TEasy Automated Pipetting System
Accuracy Siab raug: Cov cua txias tuaj yeem khaws qhov kub ntawm cov tshuaj reagent qis dua 7 ℃ rau ntau dua 60 feeb. Txhua APM raug ntsuas raws li cov txheej txheem thoob ntiaj teb, uas muaj qhov ua tau zoo dua li kev siv cov raj xa dej.
Operation Ua haujlwm yooj yim: Me me. Lub teeb yuag. Tsis xav tau cov cuab yeej xav tau rau cov hloov pauv block. Built-in PCR/qPCR txheej txheem kev npaj. Yooj yim phau ntawv ua haujlwm ntawm kev npaj tshuaj PCR.
● Daim ntawv thov dav: tuaj yeem siv rau 96/384-zoo phaj pipetting, PCR, qPCR, kev tshuaj xyuas noob thiab lwm yam kev sim siab dhau los.
FastKing Ib Kauj Ruam RT-qPCR Cov Khoom (Kuaj) (FP314)
FastKing Ib Kauj Ruam RT-qPCR Cov Khoom Siv (Soj Ntsuam) (FP314) tsim los ntawm TIANGEN yog qhov ntsuas qhov rov qab hloov pauv cov kab mob fluorescence ntau qhov ntsuas pom raws li txoj kev sojntsuam, uas yog tshwj xeeb tsim los rau kev kuaj pom cov noob hauv ntau yam qauv. KingRTase hauv cov khoom siv yog cov khoom siv hloov pauv hloov tshiab transcriptase, uas muaj zog RNA kev sib raug zoo thiab kev ruaj ntseg cua sov, nrog kev txhim kho cov ntawv sau rov qab ua tau zoo thiab txuas ntxiv lub peev xwm ntawm cov qauv txheej txheem RNA nyuaj. Kev pib kub tshiab Taq DNA polymerase kuj tseem siv los muab PCR cov tshuaj tiv thaiv kom siab dua qhov ua tau zoo thiab qhov tshwj xeeb. Ib qho ntxiv, cov khoom siv ua kom yooj yim rau cov khoom sib xyaw kom zoo tshaj plaws los ntawm kev sib xyaw Taq thiab MLV enzyme rau hauv kev sib xyaw enzyme, thiab ua ntej sib xyaw ion tsis, dNTPs, PCR stabilizer thiab txhim kho rau MasterMix, yog li ntau qhov sib xyaw ua ke cov kauj ruam tuaj yeem ua tau
yooj yim.
Efficiency Kev ua haujlwm siab: Qhov rov qab zoo transcriptase thiab DNA polymerase ua kom ntseeg tau cov tshuaj tiv thaiv tau zoo
● Kev thim rov qab zoo: Polymerase tuaj yeem nyeem los ntawm RNA tus qauv nrog cov ntsiab lus GC siab thiab cov qauv theem nrab nyuaj
Applications Cov ntawv thov dav: siv tau siab rau RNA cov qauv nrog cov khoom tsis huv los ntawm ntau hom
Sens Kev nkag siab siab: Cov qauv qis li 1 ng tuaj yeem txheeb xyuas qhov tseeb, tshwj xeeb tshaj yog rau cov qauv nplua nuj
Piv txwv rau RNA Virus Detection
Cov nucleic acid ntawm H5 avian khaub thuas tau muab rho tawm los ntawm TGuide S32 Sib Nqus Viral DNA/RNA Cov Khoom (DP604). FastKing Ib Kauj Ruam RT-qPCR Cov Khoom (Soj Ntsuam) (FP314) tau siv rau RT-qPCR kev kuaj pom siv H5 tus kab mob khaub thuas khaub thuas avian primers thiab soj ntsuam.
ABi7500Fast tau siv rau kev tshawb nrhiav RT-qPCR. Cov txiaj ntsig ntawm H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) los ntawm 200 samplesl cov qauv qhia pom tus kab mob siab nucleic acid rho tawm cov txiaj ntsig, uas tuaj yeem ua tau raws li tom ntej xav tau kev thim rov qab, PCR, RT-PCR, PCR lub sijhawm tiag tiag, thiab lwm yam. PCR lub sijhawm tiag tiag muab cov txiaj ntsig nrog kev nkag siab siab, rov ua tau zoo thiab rov ua kom zoo gradient dilution linearity. Qhov sib txawv ntawm cov kab mob nucleic acid tuaj yeem kuaj pom tseeb.
Sijhawm Tshaj Tawm: Plaub Hlis -11-2021
