TIANprep N96 Plasmid Kit

Kev tshuaj xyuas lub sijhawm tiag tiag ntawm cov xwm txheej lysis thiab kev xa tawm siab ntawm plasmids siab-purity

Cov membrane ntawm N96 Phaj CP3 hauv cov khoom siv no tau txais cov khoom tshiab silica matrix cov khoom tsim tshwj xeeb los ntawm TIANGEN, uas tuaj yeem ua tau zoo thiab tshwj xeeb tshaj yog nqus plasmid DNA, tshem tawm cov protein thiab lwm yam tsis huv rau qhov loj tshaj, yog li ua kom ntseeg tau qhov huv ntawm plasmid rho tawm. Txog li 1.3 ml ntawm daim ntawv qib siab plasmid kab mob kab lis kev cai tuaj yeem ua tiav ib qhov dej zoo, los ntawm qhov ntau txog 5-10 μg ntawm plasmid DNA tuaj yeem muab tshem tawm sai (cov txiaj ntsig cuam tshuam nrog rau yam xws li plasmid theej tus lej, kab mob hauv lub xeev, thiab lwm yam. ). Plasmid DNA rho tawm los ntawm cov khoom siv tuaj yeem siv rau hauv kev sim tshuaj lom neeg keeb kwm xws li txwv kev zom zaub mov, ua ntu zus, tshuaj xyuas lub tsev qiv ntawv, ligation thiab hloov pauv.

Miv. Tsis yog Ntim Loj
4992892 4 ua prep
4992893 24 kev npaj

Khoom nthuav dav

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Nta

■ Kev nrawm nrawm nrawm.
■ Nruab nrog TIANRed cov tshuaj tshwj xeeb, nws tuaj yeem qhia meej meej txog qib ntawm lysis ntawm cov qauv hauv cov txheej txheem rho tawm kom ntseeg tau tias ua haujlwm tau zoo thiab siab dawb huv ntawm plasmid DNA.
■ Siab dawb huv: Cov plasmid DNA uas tau lim tau tuaj yeem siv ncaj qha hauv kev sim nqes hav.
Kit Cov khoom siv no tuaj yeem siv rau hauv kev nkag siab siab siv lub tshuab ua haujlwm nucleic acid rho tawm.

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    Experimental Example Plasmid DNA tau muab rho tawm los ntawm 1 ml ntawm kev noj haus hmo ntuj Escherichia coli (TOP10) kua txiv (LB nruab nrab) siv TIANprep Highpure N96 Plasmid Kit, thiab qhov nruab nrab ntawm 5-6 μg ntawm siab purity pBS plasmid tau txais.
    Plasmid tau ntim rau hauv txhua qhov dej ntawm 1% agarose gel, thiab tau kuaj pom los ntawm electrophoresis
    Experimental Example Plasmid DNA tau muab rho tawm los ntawm 1 ml ntawm kev noj haus hmo ntuj Escherichia coli (TOP10) kua txiv (LB nruab nrab) siv TIANprep Highpure N96 Plasmid Kit, thiab qhov nruab nrab ntawm 5-6 μg ntawm siab purity pBS plasmid tau txais.
    Vector: pBS; Plasmid lim: TOP10.
    Plasmid tau ua ntu zus los ntawm ABI. PRISM 3730XL DNA sequencer.
    Sau koj cov lus ntawm no thiab xa tuaj rau peb
    Nug