TIANprep Ceev N96 Plasmid Kit

Kev nkag siab siab, tshem tawm sai ntawm cov plasmid tsawg.

Cov khoom siv txais isopropanol txheej txheem nag lossis daus kom tau txais plasmid DNA. Cov khoom siv tau txais 96-zoo lim phaj N thiab Tsis III, uas tau tsim tshwj xeeb los ntawm TIANGEN, thiab tuaj yeem nrawm thiab muaj txiaj ntsig rho tawm cov plasmid DNA siab-purity. Nws yog qhov tsim nyog rau kev npaj sai ntawm cov plasmids siab dhau. 1.3 ml ntawm daim ntawv qib siab plasmid kab mob kab lis kev cai tuaj yeem ua tiav ib qhov dej zoo, thiab 96 qhov piv txwv ntawm plasmid DNA tau muab rho tawm sai. Qhov txiaj ntsig siab tshaj plaws ntawm ib lub qhov dej yog 10-20 μg (cov txiaj ntsig cuam tshuam nrog yam xws li plasmid daim ntawv theej, kab mob hauv lub xeev, thiab lwm yam). Plasmid DNA rho tawm los ntawm cov khoom siv tuaj yeem siv rau hauv kev sim tshuaj lom neeg keeb kwm xws li txwv kev zom zaub mov, ua ntu zus, tshuaj xyuas lub tsev qiv ntawv, ligation thiab hloov pauv.

Miv. Tsis muaj:	Ntim Loj
4992868	4 cov preps
4992869	24 cov preps

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■ Yooj yim thiab yooj yim: 96 cov qauv tuaj yeem muab rho tawm hauv 2-3 teev.
■ Nruab nrog TIANRed cov tshuaj tshwj xeeb, nws tuaj yeem qhia meej meej qib ntawm lysis ntawm cov qauv hauv cov txheej txheem rho tawm kom ntseeg tau tias muaj cov plasmid DNA dawb huv.
Pur Siab dawb huv: Plasmid siab-purity tuaj yeem siv ncaj qha hauv kev sim nqes hav.

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TIANprep Rapid N96 Plasmid Kit01

	Plasmid DNA tau muab rho tawm los ntawm 1 ml ntawm kev lis kev cai ib hmos Escherichia coli (TOP10) broth (LB nruab nrab) siv TIANprep Rapid N96 Plasmid Kit, thiab qhov nruab nrab ntawm 8-10 μg ntawm siab purity pBS plasmid tau txais. 2 µl plasmid tau ntim rau hauv txhua qhov dej ntawm 1% agarose gel, thiab tau kuaj pom los ntawm electrophoresis.
	Plasmid DNA tau muab rho tawm los ntawm 1 ml ntawm kev lis kev cai ib hmos Escherichia coli (TOP10) broth (LB nruab nrab) siv TIANprep Rapid N96 Plasmid Kit, thiab qhov nruab nrab ntawm 8-10 μg ntawm siab purity pBS plasmid tau txais. Vector: pBS plasmid; Qhov siab: TOP10. Plasmid tau ua ntu zus los ntawm ABI. PRISM 3730XL DNA sequencer