DNA Bisulfite Hloov Khoom

Kev hloov pauv thiab ua kom huv tuaj yeem ua tiav hauv 2 teev, nrog kev hloov pauv mus txog 99%.

DNA Bisulfite Conversion Kit tau qhia tshwj xeeb uas tsim los rau DNA bisulfite hloov pauv hauv DNA kev tshawb fawb methylation. Kev kho cov DNA kuaj siv cov khoom siv no tuaj yeem ua tiav hauv 2 teev, thiab kev hloov pauv ntawm cytosine tsis muaj tshuaj rau uracil ntau dua 99%. Cov khoom siv no siv cov khoom tshwj xeeb ntawm DNA tus neeg sawv cev tiv thaiv, uas lav tau qhov zoo thiab rov qab los ntawm DNA tom qab hloov dua siab tshiab. Ib qho ntxiv, cov khoom siv no tseem siv txoj hauv kev tshem tawm cov kab mob sulphurous acid pawg, uas ua rau tag nrho cov txheej txheem ua haujlwm yooj yim dua.

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■ Yooj yim thiab nrawm: Kev hloov pauv thiab txheej txheem ua kom huv tuaj yeem ua tiav hauv 2 teev, nrog qhov xav tau qis rau cov twj paj nruag thiab cov cuab yeej siv, uas tsim nyog rau kev tshawb fawb hauv txhua qib.
Conversion Kev hloov pauv siab: Kev hloov pauv ntawm cytosine tsis muaj tshuaj methyl rau uracil hauv DNA qauv yog ntau dua 99%.
Sens Siab rhiab heev: Cov khoom siv no tuaj yeem ua cov qauv DNA ntawm qis li 500 pg thiab mus txog 2.5 μg.

Kev qhia tshwj xeeb

Hom:  Bisulfite Hloov dua siab tshiab
DNA qauv: 500 pg-2,5 μg
Tus nqi hloov dua siab tshiab: > 99%
Lub sijhawm ua haujlwm: ~ 2 teev
Cov ntawv thov: Cov qauv DNA ua tiav los ntawm cov khoom siv no haum rau methylation tshwj xeeb PCR/qPCR, ua ntu zus thiab microarray.

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    Table 1: Hloov pauv tus nqi ntawm "C" mus rau "U"
    Siv tib neeg cov genome los ua cov khoom siv sim, kev rov zoo ntawm cov qauv tuaj yeem ncav cuag 65%, thiab qhov sib piv ntawm cov tsis muaj tshuaj "C" rau "U" tuaj yeem ncav cuag ntau dua 99%.

    Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.

    Sau koj cov lus ntawm no thiab xa tuaj rau peb
    Nug