Cov Khoom Sib Nqus Ntshav Ntshav DNA Cov Khoom
Nta
■ Yooj yim thiab nrawm: Ultra-pure genomic DNA tuaj yeem tau txais hauv 1 teev.
Through Siab Tshaj Tawm: Cov khoom siv no tuaj yeem sib xyaw ua ke nrog cov cuab yeej siv hluav taws xob ntawm txoj kev xa cov raj thiab cov pas nrig sib nqus kom ua tiav cov txiaj ntsig siab dhau los ntawm kev sim.
■ Muaj kev nyab xeeb thiab tsis muaj tshuaj lom: Tsis xav tau cov tshuaj reagents xws li phenol/chloroform.
Pur Siab dawb huv: Cov DNA tau txais muaj lub siab dawb huv thiab tuaj yeem siv ncaj qha rau hauv kev txheeb xyuas cov nti, ua kom dhau qib sib xws thiab lwm yam kev sim.
Cov ntawv thov
DNA rov zoo los ntawm cov khoom siv tuaj yeem tsim nyog rau ntau yam kev ua haujlwm tsis tu ncua, suav nrog kev zom zaub mov, PCR, kev tsim tsev qiv ntawv, Yav qab teb blot thiab lwm yam kev sim.
Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)
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DNA tau muab rho tawm los ntawm rau daim ntawm cov ntshav qhuav qhuav swb nrog TIANGEN Sib Nqus Cov Ntshav Ntshav DNA Cov Khoom thiab cov khoom cuam tshuam los ntawm Tus Muag Khoom CW, O thiab M feem. DNA tau suav nrog 200 μl Tsis TB, thiab 5 μl tau thauj khoom rau electrophoresis. Cov txiaj ntsig tau qhia tias qhov rho tawm ntawm Cov Nqaij Sib Nqus Cov Ntshav DNA Cov Khoom Siv tau siab dua li ntawm lwm tus neeg muag khoom. M: TIANGEN Marker D15000. |
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DNA tau muab rho tawm los ntawm cov lej sib txawv ntawm cov ntshav qhuav qhuav swb los ntawm TIANGEN Magnetic Blood Spots DNA Kit. M: TIANGEN Marker D15000. Cov txiaj ntsig tau qhia tias muaj kev sib raug zoo ntawm cov ntshav qhuav nrog cov lej sib txawv. |
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DNA tau muab rho tawm los ntawm rau daim ntawm cov ntshav qhuav qhuav swb nrog TIANGEN Sib Nqus Cov Ntshav Ntshav DNA Cov Khoom thiab cov khoom cuam tshuam los ntawm Tus Muag Khoom CW, O thiab M feem. DNA tau suav nrog 200 μl Tsis TB, thiab 5 μl tau thauj khoom rau electrophoresis. Cov txiaj ntsig tau qhia tias qhov rho tawm ntawm Cov Nqaij Sib Nqus Cov Ntshav DNA Cov Khoom Siv tau siab dua li ntawm lwm tus neeg muag khoom. M: TIANGEN Marker D15000. |
A-1 Tsawg concentration ntawm cov cell lossis tus kab mob hauv qhov pib ua piv txwv -Ua kom muaj kev txaus siab ntawm cov cell lossis kab mob.
A-2 Tsis txaus lysis ntawm cov qauv-Cov qauv tsis tau muab sib xyaw kom huv nrog cov lysis tsis. Nws tau hais kom ua kom sib tov sib xyaw los ntawm mem tes-vortexing rau 1-2 zaug. -Tsis txaus lysis ntawm tes ua los ntawm kev ua haujlwm qis ntawm proteinase K. - Tsis txaus cell lysis lossis protein degradation vim lub sijhawm da dej sov tsis txaus. Nws tau hais kom txiav cov ntaub so ntswg rau hauv daim me me thiab ncua sijhawm da dej kom tshem tag nrho cov seem hauv lysate.
A-3 Tsis txaus DNA adsorption. -Tsis muaj ethanol lossis qhov feem pua tsawg dua 100% ethanol tau ntxiv ua ntej lysate tau hloov mus rau kab kem.
A-4 Tus nqi pH ntawm kev tshem tawm qhov tsis zoo heev. -Kho cov pH kom nyob nruab nrab ntawm 8.0-8.3.
Cov seem ethanol hauv cov eluent.
-Txhua yam tseem tshuav ntxuav tsis tau PW hauv qhov tsim nyog. Ethanol tuaj yeem raug tshem tawm los ntawm kev ua haujlwm centrifuging rau 3-5 feeb, thiab tom qab ntawd muab tso rau ntawm chav sov lossis 50 ℃ qhov cub rau 1-2 feeb.
A-1 Cov qauv tsis tshiab. - Txheeb tawm qhov piv txwv DNA zoo raws li kev tswj hwm txhawm rau txiav txim siab tias DNA hauv cov qauv tau puas lawm.
A-2 Kev kho ua ntej tsis raug. -Caused los ntawm cov kua nitrogen sib tsoo ntau dhau, rov ua kom muaj dej noo, lossis ntau dhau ntawm cov qauv.
Kev kho ua ntej yuav tsum sib txawv rau cov qauv sib txawv. Rau kev kuaj cov nroj tsuag, nco ntsoov ua tib zoo zom hauv cov kua nitrogen. Rau kev kuaj tsiaj, homogenate lossis ua tib zoo zom hauv cov kua nitrogen. Txog kev kuaj nrog cov phab ntsa ntawm tes uas nyuaj rau tawg, xws li G+ cov kab mob thiab cov poov xab, nws tau hais kom siv lysozyme, lyticase lossis cov txheej txheem kho tshuab txhawm rau txhawm rau phab ntsa ntawm tes.
4992201/4992202 Plant Genomic DNA Kit tau txais cov txheej txheem raws kab uas xav tau chloroform rau kev rho tawm. Nws yog qhov tshwj xeeb tshaj yog haum rau ntau yam piv txwv cog, ntxiv rau cog cov hmoov qhuav. Hi-DNAsecure Plant Kit tseem yog kem-raws, tab sis tsis xav tau phenol/chloroform rho tawm, ua rau nws nyab xeeb thiab tsis muaj tshuaj lom. Nws yog qhov tsim nyog rau cov nroj tsuag uas muaj polysaccharides siab thiab cov ntsiab lus polyphenol. 4992709/4992710 DNAquick Plant System tau txais cov txheej txheem ua kua. Phenol/chloroform rho tawm tsis xav tau ib yam nkaus. Cov txheej txheem kev ua kom huv yog yooj yim thiab nrawm nrog tsis muaj kev txwv rau qhov pib ua piv txwv, yog li cov neeg siv tuaj yeem hloov kho qhov yoog raws qhov xav tau ntawm kev sim. Loj me me ntawm gDNA cov khoom tawg tuaj yeem tau txais nrog cov txiaj ntsig siab.
Lub genomic DNA tau muab rho tawm los ntawm ntau qhov sib txawv ntawm tib neeg cov ntshav tag nrho los ntawm TIANamp Blood DNA Kit. Cov txiaj ntsig yog raws li hauv qab no. Cov txiaj ntsig tau teev tseg raws li siv nkaus xwb, qhov txiaj ntsig rho tawm tiag tiag nyob ntawm qhov xwm txheej ntawm kev kuaj.
Cov ntshav khov DNA rho tawm tuaj yeem ua tiav siv cov tshuaj reagents muab hauv ob qho khoom siv no los ntawm kev hloov pauv cov txheej txheem tshwj xeeb rau kev qhia tshwj xeeb rau cov ntshav khov DNA tshem tawm. Daim ntawv mos ntawm cov ntshav khov DNA cov txheej txheem rho tawm tuaj yeem muab tawm thaum thov.
Nthuav cov qauv tshiab nrog 1 ml PBS, cov kua ntsev ib txwm lossis TE tsis. Ua kom tiav cov homogenize cov qauv los ntawm homogenizer thiab sau cov nag mus rau hauv qab ntawm lub raj los ntawm kev ua centrifuging. Muab cov supernatant tso tseg, thiab rov ua kom cov nag los nrog 200 μl tsis GA. Kev ua kom huv DNA hauv qab no tuaj yeem ua raws li cov lus qhia.
Txog kev ua kom huv ntawm gDNA hauv ntshav, ntshav thiab kuaj lub cev, TIANamp Micro DNA Kit tau pom zoo. Txog kev ua kom huv ntawm tus kab mob gDNA los ntawm kev kuaj ntshav/ntshav, TIANamp Virus DNA/RNA Kit tau pom zoo. Txog kev ua kom huv ntawm cov kab mob gDNA los ntawm cov ntshav thiab cov ntshav ntshav, TIANamp Cov Kab Mob Cov Kab Mob DNA tau pom zoo (lysozyme yuav tsum suav nrog rau cov kab mob zoo). Txog cov hnoos qeev, Hi-Swab DNA Kit thiab TIANamp Bacteria DNA Kit tau pom zoo.
DNAsecure Plant Kit lossis DNAquick Plant System tau pom zoo rau kev tshem tawm cov noob genome. Txog cov poov xab genome rho tawm, TIANamp Poov Cov Khoom DNA tau pom zoo (lyticase yuav tsum tau npaj tus kheej).
Pawg khoom
Vim Li Cas Xaiv Peb
Txij li nws tau tsim, peb lub Hoobkas tau tsim thawj cov khoom lag luam hauv ntiaj teb nrog ua raws txoj cai
zoo ua ntej. Peb cov khoom tau txais txiaj ntsig zoo hauv kev lag luam thiab muaj txiaj ntsig zoo ntawm cov neeg siv khoom qub thiab qub ..
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