TGuide FFPE DNA One-Step Kit

Ib kauj ruam rho tawm ntawm genome DNA los ntawm FFPE cov qauv.

TGuide FFPE DNA One-Step Kit tau qhia tshwj xeeb uas tsim los rho tawm genome DNA los ntawm cov ntaub so ntswg paraffin nrog TGuide automated nucleic acid extractors. Cov khoom siv no siv cov txheej txheem cua sov ib kauj ruam, yog li kev ua kom tsis zoo thiab cov ntaub so ntswg tuaj yeem ua tiav ib txhij, thiab cov tshuaj tsis zoo xws li xylene tsis muaj. Cov khoom siv no muaj ob qhov kev xaiv rho tawm: Rau qhov ntsuas me me, xaiv 2 teev lysis; Rau qhov ntau ntawm cov qauv, xaiv 16 teev hmo ntuj lysis. Cov khoom siv txais cov cellulose kos cov hlau nplaum sib nqus thev naus laus zis kom tshem tawm cov genome DNA.

Miv. Tsis yog	Ntim Loj
OS-M405	48 cov preps

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Cov ntawv thov

Purified DNA tuaj yeem siv ncaj qha hauv ntau txoj haujlwm ua haujlwm, suav nrog kev zom zaub mov, PCR, kev tsim tsev qiv ntawv, Yav qab teb blot thiab lwm yam kev sim.

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Ara Kev siv lub tshuab ua kom tsis zoo: Kev tshem tawm thiab tshem tawm cov theem tuaj yeem ua tiav tau yooj yim los ntawm kev muab cov tshuaj paraffin kho tsis tau tso rau hauv cov tshuaj reagent thiab tom qab ntawd ntxiv Sula Roj thiab Proteinase K.
Results Cov txiaj ntsig tau ntseeg tau: Cov genome DNA tau txais dawb los ntawm RNA thiab kev sib kis ntawm cov protein, thiab tuaj yeem siv ncaj qha rau PCR lossis fluorescence quantitative PCR.
■ Muaj kev nyab xeeb thiab tsis muaj mob: Cov kuab tshuaj organic uas muaj teeb meem rau tib neeg lub cev xws li phenol chloroform tsis xav tau.

Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)

Yav dhau los: TGuide Cells/Tissue Genomic DNA Kit

Tom ntej: TGuide Bacteria Genomic DNA Kit

Cov txiaj ntsig tau pom tias rau qhov ntim ntau qhov piv txwv, cov txiaj ntsig rho tawm ntawm ob txoj hauv kev tau sib piv; rau cov qauv nrog qhov nruab nrab thiab ntim me me, cov txiaj ntsig ntawm ib qib kev ua kom tsis zoo (ua nrog TGuide) yog ntau dua li ntawm cov txheej txheem kev ua kom tsis zoo. Yog li ntawd, ib qib zuj zus txoj kev ua kom tsis zoo tsuas yog ua kom yooj yim rau kev ua haujlwm, txo qhov kev pheej hmoo, tab sis tseem txhim kho kev ua kom tau zoo dua qub.
Nco tseg:
Cov ntim loj piv txwv: thaj tsam ntu tsis pub dhau 2.5-4 cm² thiab cov tuab yog hauv 5-20 μm.
Cov ntim ntim nruab nrab: thaj tsam ntu yog nyob hauv 1.5-2.5 cm² thiab cov tuab yog hauv 5-20 μm.
Cov ntim me me piv txwv: thaj tsam ntu tsis pub dhau 0.2-1.5 cm² thiab cov tuab yog hauv 5-20 μm.

Q: Me me lossis tsis muaj DNA hauv qhov tsim nyog.

A-1 Tsawg concentration ntawm cov cell lossis tus kab mob hauv qhov pib ua piv txwv -Ua kom muaj kev txaus siab ntawm cov cell lossis kab mob.

A-2 Tsis txaus lysis ntawm cov qauv-Cov qauv tsis tau muab sib xyaw kom huv nrog cov lysis tsis. Nws tau hais kom ua kom sib tov sib xyaw los ntawm mem tes-vortexing rau 1-2 zaug. -Tsis txaus lysis ntawm tes ua los ntawm kev ua haujlwm qis ntawm proteinase K. - Tsis txaus cell lysis lossis protein degradation vim lub sijhawm da dej sov tsis txaus. Nws tau hais kom txiav cov ntaub so ntswg rau hauv daim me me thiab ncua sijhawm da dej kom tshem tag nrho cov seem hauv lysate.

A-3 Tsis txaus DNA adsorption. -Tsis muaj ethanol lossis qhov feem pua tsawg dua 100% ethanol tau ntxiv ua ntej lysate tau hloov mus rau kab kem.

A-4 Tus nqi pH ntawm kev tshem tawm qhov tsis zoo heev. -Kho cov pH kom nyob nruab nrab ntawm 8.0-8.3.

Q: DNA tsis ua tau zoo hauv kev sim tshuaj enzymatic nqes hav.

Cov seem ethanol hauv cov eluent.

-Txhua yam tseem tshuav ntxuav tsis tau PW hauv qhov tsim nyog. Ethanol tuaj yeem raug tshem tawm los ntawm kev ua haujlwm centrifuging rau 3-5 feeb, thiab tom qab ntawd muab tso rau ntawm chav sov lossis 50 ℃ qhov cub rau 1-2 feeb.

Q: DNA puas tsuaj

A-1 Cov qauv tsis tshiab. - Txheeb tawm qhov piv txwv DNA zoo raws li kev tswj hwm txhawm rau txiav txim siab tias DNA hauv cov qauv tau puas lawm.

A-2 Kev kho ua ntej tsis raug. -Caused los ntawm cov kua nitrogen sib tsoo ntau dhau, rov ua kom muaj dej noo, lossis ntau dhau ntawm cov qauv.

Q: Yuav ua li cas thiaj ua tau qhov pretreatment rau gDNA rho tawm?

Kev kho ua ntej yuav tsum sib txawv rau cov qauv sib txawv. Rau kev kuaj cov nroj tsuag, nco ntsoov ua tib zoo zom hauv cov kua nitrogen. Rau kev kuaj tsiaj, homogenate lossis ua tib zoo zom hauv cov kua nitrogen. Txog kev kuaj nrog cov phab ntsa ntawm tes uas nyuaj rau tawg, xws li G+ cov kab mob thiab cov poov xab, nws tau hais kom siv lysozyme, lyticase lossis cov txheej txheem kho tshuab txhawm rau txhawm rau phab ntsa ntawm tes.

Q: Dab tsi yog qhov sib txawv ntawm peb tsob nroj gDNA cov khoom siv rho tawm 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit tau txais cov txheej txheem raws kab uas xav tau chloroform rau kev rho tawm. Nws yog qhov tshwj xeeb tshaj yog haum rau ntau yam piv txwv cog, ntxiv rau cog cov hmoov qhuav. Hi-DNAsecure Plant Kit tseem yog kem-raws, tab sis tsis xav tau phenol/chloroform rho tawm, ua rau nws nyab xeeb thiab tsis muaj tshuaj lom. Nws yog qhov tsim nyog rau cov nroj tsuag uas muaj polysaccharides siab thiab cov ntsiab lus polyphenol. 4992709/4992710 DNAquick Plant System tau txais cov txheej txheem ua kua. Phenol/chloroform rho tawm tsis xav tau ib yam nkaus. Cov txheej txheem kev ua kom huv yog yooj yim thiab nrawm nrog tsis muaj kev txwv rau qhov pib ua piv txwv, yog li cov neeg siv tuaj yeem hloov kho qhov yoog raws qhov xav tau ntawm kev sim. Loj me me ntawm gDNA cov khoom tawg tuaj yeem tau txais nrog cov txiaj ntsig siab.

Dab tsi yog qhov kwv yees kwv yees ntawm gDNA los ntawm 1 ml ntshav coj los ntawm TIANamp Cov Ntshav DNA Cov Khoom?

Lub genomic DNA tau muab rho tawm los ntawm ntau qhov sib txawv ntawm tib neeg cov ntshav tag nrho los ntawm TIANamp Blood DNA Kit. Cov txiaj ntsig yog raws li hauv qab no. Cov txiaj ntsig tau teev tseg raws li siv nkaus xwb, qhov txiaj ntsig rho tawm tiag tiag nyob ntawm qhov xwm txheej ntawm kev kuaj.

Q: Puas yog 4992207/4992208 thiab 4992722/4992723 tuaj yeem siv los tshem tawm cov ntshav txhaws DNA?

Cov ntshav khov DNA rho tawm tuaj yeem ua tiav siv cov tshuaj reagents muab hauv ob qho khoom siv no los ntawm kev hloov pauv cov txheej txheem tshwj xeeb rau kev qhia tshwj xeeb rau cov ntshav khov DNA tshem tawm. Daim ntawv mos ntawm cov ntshav khov DNA cov txheej txheem rho tawm tuaj yeem muab tawm thaum thov.

Q: Thaum thov TIANamp Genomic DNA Kit, yuav ua li cas txhawm rau cov ntaub so ntswg tshiab rau hauv kev tshem tawm ntawm tes?

Nthuav cov qauv tshiab nrog 1 ml PBS, cov kua ntsev ib txwm lossis TE tsis. Ua kom tiav cov homogenize cov qauv los ntawm homogenizer thiab sau cov nag mus rau hauv qab ntawm lub raj los ntawm kev ua centrifuging. Muab cov supernatant tso tseg, thiab rov ua kom cov nag los nrog 200 μl tsis GA. Kev ua kom huv DNA hauv qab no tuaj yeem ua raws li cov lus qhia.

Q: Yuav ua li cas xaiv cov khoom lag luam rau kev tshem tawm DNA los ntawm ntshav, ntshav thiab kuaj lub cev?

Txog kev ua kom huv ntawm gDNA hauv ntshav, ntshav thiab kuaj lub cev, TIANamp Micro DNA Kit tau pom zoo. Txog kev ua kom huv ntawm tus kab mob gDNA los ntawm kev kuaj ntshav/ntshav, TIANamp Virus DNA/RNA Kit tau pom zoo. Txog kev ua kom huv ntawm cov kab mob gDNA los ntawm cov ntshav thiab cov ntshav ntshav, TIANamp Cov Kab Mob Cov Kab Mob DNA tau pom zoo (lysozyme yuav tsum suav nrog rau cov kab mob zoo). Txog cov hnoos qeev, Hi-Swab DNA Kit thiab TIANamp Bacteria DNA Kit tau pom zoo.

Q: Yuav ua li cas xaiv cov khoom siv rau gDNA rho tawm los ntawm kev kuaj cov pwm?

DNAsecure Plant Kit lossis DNAquick Plant System tau pom zoo rau kev tshem tawm cov noob genome. Txog cov poov xab genome rho tawm, TIANamp Poov Cov Khoom DNA tau pom zoo (lyticase yuav tsum tau npaj tus kheej).

Cell Dawb Dna Extraction Kit