TGuide Virus DNA/RNA Kit
Cov ntawv thov
Purified nucleic acid yog tsim rau siab rhiab heev PCR, RTPCR, PCR ntau, ntau RT-PCR thiab lwm yam kev sim. Cov khoom siv tau raug txheeb xyuas los ntawm nqes hav nrhiav HBV, HCV, HIV thiab kab mob khaub thuas.
Nta
■ Kev rho tawm yooj yim thiab nrawm: TGuide cov khoom siv ntxiv yog ua raws lub hauv paus ntsiab lus ntawm nucleic acid purification los ntawm cov hlaws sib nqus, thiab cov txheej txheem kis nucleic acid rho tawm tuaj yeem ua tiav hauv 57 min.
■ Tsis muaj kev sib kis: Kev ywj pheej ntim cov tshuaj reagent tau ntim ua ntej los tiv thaiv kev sib kis.
Yield Cov txiaj ntsig tau zoo: Cov khoom siv muaj Carrier RNA zoo los txhim kho kev ua haujlwm ntawm nucleic acid ntes.
■ Tsis muaj phenol thiab chloroform rho tawm: Tsis xav tau cov organic hnyav ua rau tib neeg lub cev xws li phenol thiab chloroform xav tau.
■ Cov qauv piv txwv yooj yim: 200 μl/400 μl cov qauv tuaj yeem muab ncaj qha rho tawm nrog cov khoom siv sib xws.
Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)
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Fluorescence quantitative PCR kuaj pom ntawm HBV Daim duab 1: Ua kom huv 200 μl cov neeg mob ntshav qab zib muaj HBV ntawm qhov sib txawv sib txawv siv TGuide Tus Kab Mob DNA/RNA Cov Khoom. Qhov tau txais HBV viral DNA tau yaj hauv 60 μl elution buffer. |
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Daim duab 2: Tshem tawm cov ntshav kuaj uas muaj ntau qhov sib txawv ntawm HCV tus kab mob los ntawm TGuide Tus Kab Mob DNA/RNA Cov Khoom, thiab kuaj pom qhov tshwm sim los ntawm PCR. 1: 5 × 100 HCV Serum 2: 5 × 101 HCV Serum tau 3: 5 × 10 hli2 HCV Serum 4: 5 × 103 HCV Serum tau 5: 5 × 104 HCV Serum 6: 5 × 105 HCV Serum tau P: Tswj zoo N: Kev tswj tsis zoo M: 100 bp DNA ntaiv |
A-1 Tsawg concentration ntawm cov cell lossis tus kab mob hauv qhov pib ua piv txwv -Ua kom muaj kev txaus siab ntawm cov cell lossis kab mob.
A-2 Tsis txaus lysis ntawm cov qauv-Cov qauv tsis tau muab sib xyaw kom huv nrog cov lysis tsis. Nws tau hais kom ua kom sib tov sib xyaw los ntawm mem tes-vortexing rau 1-2 zaug. -Tsis txaus lysis ntawm tes ua los ntawm kev ua haujlwm qis ntawm proteinase K. - Tsis txaus cell lysis lossis protein degradation vim lub sijhawm da dej sov tsis txaus. Nws tau hais kom txiav cov ntaub so ntswg rau hauv daim me me thiab ncua sijhawm da dej kom tshem tag nrho cov seem hauv lysate.
A-3 Tsis txaus DNA adsorption. -Tsis muaj ethanol lossis qhov feem pua tsawg dua 100% ethanol tau ntxiv ua ntej lysate tau hloov mus rau kab kem.
A-4 Tus nqi pH ntawm kev tshem tawm qhov tsis zoo heev. -Kho cov pH kom nyob nruab nrab ntawm 8.0-8.3.
Cov seem ethanol hauv cov eluent.
-Txhua yam tseem tshuav ntxuav tsis tau PW hauv qhov tsim nyog. Ethanol tuaj yeem raug tshem tawm los ntawm kev ua haujlwm centrifuging rau 3-5 feeb, thiab tom qab ntawd muab tso rau ntawm chav sov lossis 50 ℃ qhov cub rau 1-2 feeb.
A-1 Cov qauv tsis tshiab. - Txheeb tawm qhov piv txwv DNA zoo raws li kev tswj hwm txhawm rau txiav txim siab tias DNA hauv cov qauv tau puas lawm.
A-2 Kev kho ua ntej tsis raug. -Caused los ntawm cov kua nitrogen sib tsoo ntau dhau, rov ua kom muaj dej noo, lossis ntau dhau ntawm cov qauv.
Kev kho ua ntej yuav tsum sib txawv rau cov qauv sib txawv. Rau kev kuaj cov nroj tsuag, nco ntsoov ua tib zoo zom hauv cov kua nitrogen. Rau kev kuaj tsiaj, homogenate lossis ua tib zoo zom hauv cov kua nitrogen. Txog kev kuaj nrog cov phab ntsa ntawm tes uas nyuaj rau tawg, xws li G+ cov kab mob thiab cov poov xab, nws tau hais kom siv lysozyme, lyticase lossis cov txheej txheem kho tshuab txhawm rau txhawm rau phab ntsa ntawm tes.
4992201/4992202 Plant Genomic DNA Kit tau txais cov txheej txheem raws kab uas xav tau chloroform rau kev rho tawm. Nws yog qhov tshwj xeeb tshaj yog haum rau ntau yam piv txwv cog, ntxiv rau cog cov hmoov qhuav. Hi-DNAsecure Plant Kit tseem yog kem-raws, tab sis tsis xav tau phenol/chloroform rho tawm, ua rau nws nyab xeeb thiab tsis muaj tshuaj lom. Nws yog qhov tsim nyog rau cov nroj tsuag uas muaj polysaccharides siab thiab cov ntsiab lus polyphenol. 4992709/4992710 DNAquick Plant System tau txais cov txheej txheem ua kua. Phenol/chloroform rho tawm tsis xav tau ib yam nkaus. Cov txheej txheem kev ua kom huv yog yooj yim thiab nrawm nrog tsis muaj kev txwv rau qhov pib ua piv txwv, yog li cov neeg siv tuaj yeem hloov kho qhov yoog raws qhov xav tau ntawm kev sim. Loj me me ntawm gDNA cov khoom tawg tuaj yeem tau txais nrog cov txiaj ntsig siab.
Lub genomic DNA tau muab rho tawm los ntawm ntau qhov sib txawv ntawm tib neeg cov ntshav tag nrho los ntawm TIANamp Blood DNA Kit. Cov txiaj ntsig yog raws li hauv qab no. Cov txiaj ntsig tau teev tseg raws li siv nkaus xwb, qhov txiaj ntsig rho tawm tiag tiag nyob ntawm qhov xwm txheej ntawm kev kuaj.
Cov ntshav khov DNA rho tawm tuaj yeem ua tiav siv cov tshuaj reagents muab hauv ob qho khoom siv no los ntawm kev hloov pauv cov txheej txheem tshwj xeeb rau kev qhia tshwj xeeb rau cov ntshav khov DNA tshem tawm. Daim ntawv mos ntawm cov ntshav khov DNA cov txheej txheem rho tawm tuaj yeem muab tawm thaum thov.
Nthuav cov qauv tshiab nrog 1 ml PBS, cov kua ntsev ib txwm lossis TE tsis. Ua kom tiav cov homogenize cov qauv los ntawm homogenizer thiab sau cov nag mus rau hauv qab ntawm lub raj los ntawm kev ua centrifuging. Muab cov supernatant tso tseg, thiab rov ua kom cov nag los nrog 200 μl tsis GA. Kev ua kom huv DNA hauv qab no tuaj yeem ua raws li cov lus qhia.
Txog kev ua kom huv ntawm gDNA hauv ntshav, ntshav thiab kuaj lub cev, TIANamp Micro DNA Kit tau pom zoo. Txog kev ua kom huv ntawm tus kab mob gDNA los ntawm kev kuaj ntshav/ntshav, TIANamp Virus DNA/RNA Kit tau pom zoo. Txog kev ua kom huv ntawm cov kab mob gDNA los ntawm cov ntshav thiab cov ntshav ntshav, TIANamp Cov Kab Mob Cov Kab Mob DNA tau pom zoo (lysozyme yuav tsum suav nrog rau cov kab mob zoo). Txog cov hnoos qeev, Hi-Swab DNA Kit thiab TIANamp Bacteria DNA Kit tau pom zoo.
DNAsecure Plant Kit lossis DNAquick Plant System tau pom zoo rau kev tshem tawm cov noob genome. Txog cov poov xab genome rho tawm, TIANamp Poov Cov Khoom DNA tau pom zoo (lyticase yuav tsum tau npaj tus kheej).
Pawg khoom
Vim Li Cas Xaiv Peb
Txij li nws tau tsim, peb lub Hoobkas tau tsim thawj cov khoom lag luam hauv ntiaj teb nrog ua raws txoj cai
zoo ua ntej. Peb cov khoom tau txais txiaj ntsig zoo hauv kev lag luam thiab muaj txiaj ntsig zoo ntawm cov neeg siv khoom qub thiab qub ..
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