TIANamp Cov Ntshav DNA Cov Khoom
Nta
Pur High purity: Silica membrane tau tshem ntawm feem ntau impurities.
■ Kev siv dav: Haum rau cov ntshav tiv thaiv coagulant (EDTA, heparin thiab lwm yam), lub tsho me me thiab cov ntshav txhaws.
Operation Kev ua haujlwm nrawm: Genomic DNA tuaj yeem tau txais hauv 1 teev.
Kev qhia tshwj xeeb
Hom: Spin kem raws
Qauv thiab pib ntim: 100-1000 μl tag nrho cov ntshav los ntawm tsiaj txhu, 5-20 μl tag nrho cov ntshav los ntawm noog lossis amphibian, 200-1000 μl ntshav txhaws
Lub Hom Phiaj: Genomic DNA.
Qhov hnyav: 3-40 μg
Lub sijhawm ua haujlwm: ~ 1 teev
Cov ntawv thov nqes hav: Txwv kev zom zaub mov, tshuaj PCR, Yav qab teb blot, nti sib xyaw, tsim tsev qiv ntawv NGS, thiab lwm yam.
Cov Lus Tseem Ceeb
1. Txhawm rau tshem RNA qhov seem, RNase A tov (100 mg/ml) yuav tsum tau npaj rau tus kheej.
2. Cov khoom no tau sau npe ua chav kawm I hauv vitrokuaj mob reagent los ntawm Beijing FDA. Thov hu rau TIANGEN yog xav paub ntxiv.
Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)
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Genomic DNA tau muab rho tawm los ntawm kev kuaj ntshav siv TIANamp Blood DNA Kit. M: TIANGEN Marker D15000. Piv txwv: 200 μl tib neeg cov ntshav coagulation nrog EDTA, EDTA/NaF, heparin thiab sodium citrate; lub tsho tiv no los ntawm 2 ml ntshav; 10 μl ntshav qaib thiab 200 μl nas ntshav. Rau lub tsho tiv no, 2 μl ntawm 100 μl eluates tau thauj khoom; rau lwm tus, 4 μl ntawm 100 μl eluates tau thauj khoom ib txoj kab. Cov txiaj ntsig sim: TIANamp Cov Khoom Siv DNA Ntshav tuaj yeem siv dav hauv qhov ua tau zoo DNA kev ua kom huv los ntawm tib neeg anticoagulants nrog kev kho mob sib txawv nrog rau cov ntshav los ntawm ntau hom. |
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Kev sib piv ntawm cov genomic DNA rho tawm los ntawm 200 μl tib neeg anticoagulation nrog EDTA siv TIANamp Ntshav DNA Cov Khoom thiab cov khoom zoo sib xws los ntawm lwm tus neeg muag khoom. M: TIANGEN Marker D15000. 4 μl ntawm 100 μl eluates tau thauj khoom ib txoj kab. Cov txiaj ntsig sim: Sib piv rau lwm tus neeg muab khoom, TIANamp Cov DNA DNA Cov Khoom tuaj yeem tau txais txiaj ntsig siab dua ntawm cov ntshav gDNA. |
A-1 Tsawg concentration ntawm cov cell lossis tus kab mob hauv qhov pib ua piv txwv -Ua kom muaj kev txaus siab ntawm cov cell lossis kab mob.
A-2 Tsis txaus lysis ntawm cov qauv-Cov qauv tsis tau muab sib xyaw kom huv nrog cov lysis tsis. Nws tau hais kom ua kom sib tov sib xyaw los ntawm mem tes-vortexing rau 1-2 zaug. -Tsis txaus lysis ntawm tes ua los ntawm kev ua haujlwm qis ntawm proteinase K. - Tsis txaus cell lysis lossis protein degradation vim lub sijhawm da dej sov tsis txaus. Nws tau hais kom txiav cov ntaub so ntswg rau hauv daim me me thiab ncua sijhawm da dej kom tshem tag nrho cov seem hauv lysate.
A-3 Tsis txaus DNA adsorption. -Tsis muaj ethanol lossis qhov feem pua tsawg dua 100% ethanol tau ntxiv ua ntej lysate tau hloov mus rau kab kem.
A-4 Tus nqi pH ntawm kev tshem tawm qhov tsis zoo heev. -Kho cov pH kom nyob nruab nrab ntawm 8.0-8.3.
Cov seem ethanol hauv cov eluent.
-Txhua yam tseem tshuav ntxuav tsis tau PW hauv qhov tsim nyog. Ethanol tuaj yeem raug tshem tawm los ntawm kev ua haujlwm centrifuging rau 3-5 feeb, thiab tom qab ntawd muab tso rau ntawm chav sov lossis 50 ℃ qhov cub rau 1-2 feeb.
A-1 Cov qauv tsis tshiab. - Txheeb tawm qhov piv txwv DNA zoo raws li kev tswj hwm txhawm rau txiav txim siab tias DNA hauv cov qauv tau puas lawm.
A-2 Kev kho ua ntej tsis raug. -Caused los ntawm cov kua nitrogen sib tsoo ntau dhau, rov ua kom muaj dej noo, lossis ntau dhau ntawm cov qauv.
Kev kho ua ntej yuav tsum sib txawv rau cov qauv sib txawv. Rau kev kuaj cov nroj tsuag, nco ntsoov ua tib zoo zom hauv cov kua nitrogen. Rau kev kuaj tsiaj, homogenate lossis ua tib zoo zom hauv cov kua nitrogen. Txog kev kuaj nrog cov phab ntsa ntawm tes uas nyuaj rau tawg, xws li G+ cov kab mob thiab cov poov xab, nws tau hais kom siv lysozyme, lyticase lossis cov txheej txheem kho tshuab txhawm rau txhawm rau phab ntsa ntawm tes.
4992201/4992202 Plant Genomic DNA Kit tau txais cov txheej txheem raws kab uas xav tau chloroform rau kev rho tawm. Nws yog qhov tshwj xeeb tshaj yog haum rau ntau yam piv txwv cog, ntxiv rau cog cov hmoov qhuav. Hi-DNAsecure Plant Kit tseem yog kem-raws, tab sis tsis xav tau phenol/chloroform rho tawm, ua rau nws nyab xeeb thiab tsis muaj tshuaj lom. Nws yog qhov tsim nyog rau cov nroj tsuag uas muaj polysaccharides siab thiab cov ntsiab lus polyphenol. 4992709/4992710 DNAquick Plant System tau txais cov txheej txheem ua kua. Phenol/chloroform rho tawm tsis xav tau ib yam nkaus. Cov txheej txheem kev ua kom huv yog yooj yim thiab nrawm nrog tsis muaj kev txwv rau qhov pib ua piv txwv, yog li cov neeg siv tuaj yeem hloov kho qhov yoog raws qhov xav tau ntawm kev sim. Loj me me ntawm gDNA cov khoom tawg tuaj yeem tau txais nrog cov txiaj ntsig siab.
Lub genomic DNA tau muab rho tawm los ntawm ntau qhov sib txawv ntawm tib neeg cov ntshav tag nrho los ntawm TIANamp Blood DNA Kit. Cov txiaj ntsig yog raws li hauv qab no. Cov txiaj ntsig tau teev tseg raws li siv nkaus xwb, qhov txiaj ntsig rho tawm tiag tiag nyob ntawm qhov xwm txheej ntawm kev kuaj.
Cov ntshav khov DNA rho tawm tuaj yeem ua tiav siv cov tshuaj reagents muab hauv ob qho khoom siv no los ntawm kev hloov pauv cov txheej txheem tshwj xeeb rau kev qhia tshwj xeeb rau cov ntshav khov DNA tshem tawm. Daim ntawv mos ntawm cov ntshav khov DNA cov txheej txheem rho tawm tuaj yeem muab tawm thaum thov.
Nthuav cov qauv tshiab nrog 1 ml PBS, cov kua ntsev ib txwm lossis TE tsis. Ua kom tiav cov homogenize cov qauv los ntawm homogenizer thiab sau cov nag mus rau hauv qab ntawm ib lub raj los ntawm centrifuging. Muab cov supernatant tso tseg, thiab rov ua kom cov nag los nrog 200 μl tsis GA. Kev ua kom huv DNA hauv qab no tuaj yeem ua raws li cov lus qhia.
Txog kev ua kom huv ntawm gDNA hauv ntshav, ntshav thiab kuaj lub cev, TIANamp Micro DNA Kit tau pom zoo. Txog kev ua kom huv ntawm tus kab mob gDNA los ntawm kev kuaj ntshav/ntshav, TIANamp Virus DNA/RNA Kit tau pom zoo. Txog kev ua kom huv ntawm cov kab mob gDNA los ntawm cov ntshav thiab cov ntshav ntshav, TIANamp Cov Kab Mob Cov Kab Mob DNA tau pom zoo (lysozyme yuav tsum suav nrog rau cov kab mob zoo). Txog cov hnoos qeev, Hi-Swab DNA Kit thiab TIANamp Bacteria DNA Kit tau pom zoo.
DNAsecure Plant Kit lossis DNAquick Plant System tau pom zoo rau kev tshem tawm cov noob genome. Txog cov poov xab genome rho tawm, TIANamp Poov Cov Khoom DNA tau pom zoo (lyticase yuav tsum tau npaj tus kheej).
Pawg khoom
Vim Li Cas Xaiv Peb
Txij li nws tau tsim, peb lub Hoobkas tau tsim thawj cov khoom lag luam hauv ntiaj teb nrog ua raws txoj cai
zoo ua ntej. Peb cov khoom tau txais txiaj ntsig zoo hauv kev lag luam thiab muaj txiaj ntsig zoo ntawm cov neeg siv khoom qub thiab qub ..
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