EndoFree Mini Plasmid Kit II

Rau kev ua kom huv ntawm qib endotoxin-dawb kis plasmid DNA.

Qhov EndoFree Mini Plasmid Kit II tau txais lub cim tshwj xeeb silica membrane adsorption thev naus laus zis kom ua tau zoo thiab tshwj xeeb khi plasmid DNA. Qhov tshwj xeeb endotoxin-tshem tawm cov txheej txheem tuaj yeem tshem tawm cov khoom tsis huv xws li endotoxin thiab cov protein. Tag nrho cov txheej txheem rho tawm tsuas yog siv sijhawm 1 teev, uas yooj yim thiab nrawm. Lub plasmid rho tawm cov txiaj ntsig cuam tshuam nrog plasmid daim ntawv theej, tus kab mob hom kab mob thiab kab lis kev cai. Txog li 70 μg ntawm plasmid DNA tuaj yeem muab rho tawm los ntawm 5-15 ml ntawm cov kab lis kev cai ib txwm muaj kab mob kab lis kev cai nruab nrab.

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4992422 50 ua prep

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Nta

■ Ceev ceev thiab tau txais txiaj ntsig zoo: 5-70 μg plasmid DNA tuaj yeem muab rho tawm nyob ib ncig ntawm 1 teev.
■ High-purity: Qhov tshwj xeeb endotoxin tshem tawm qhov system ua ke nrog Spin Column CP4 tuaj yeem tshwj xeeb nqus cov nucleic acid thiab tshem tawm endotoxin mus rau qib tsawg.
Range Ntau yam kev siv: Tsim nyog rau kev sim ib txwm xws li txwv tsis pub zom zaub mov, PCR, ua ntu zus, ligation, hloov pauv, ntxiv rau qhov kev sim siab zoo xws li kev kho noob, cell microinjection, noob ntsiag to, hloov pauv, thiab lwm yam.

Plasmid Yield

Plasmid Yield

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    Experimental Example Haum rau kev rho tawm ntawm cov ntawv plasmids siab/qis
    Daim duab 1. Kev tshem tawm plasmid DNA los ntawm ntau yam ntim kab mob kab lis kev cai siv EndoFree Mini Plasmid Kit II.
    Kev tshem tawm ntim: 200 μl; Chaw thau khoom ntim: 3 μl cov ntawv qis plasmid pBR322, 2 μl daim ntawv plasmid pBS siab.
    Cov electrophoresis tau ua ntawm 6 V/cm rau 30 min ntawm 1% agarose.
    Kev hloov pauv ntawm tes los ntawm cov plasmid purified
    Experimental Example Daim duab 2 PEGFP plasmid purified los ntawm EndoFree Mini Plasmid Kit II tau kis mus rau 293T hlwb. Kev nthuav tawm ntawm GFP tau tshawb pom hauv 48 teev tom qab kis mob
    Sau koj cov lus ntawm no thiab xa tuaj rau peb
    Nug