TIANprep Rapid Mini Plasmid Kit

Rau kev ntxuav sai ntawm plasmid DNA ntawm qib molecular biology los ntawm cov txheej txheem alkaline lysis.

TIANprep Rapid Mini Plasmid Kit tau ua kom zoo dua qub los ntawm kev siv tshuab alkaline lysis, los ntawm qhov zoo plasmid DNA tuaj yeem ua kom huv hauv 8 feeb. Qhov tshiab lysis tsis pub tso cai adsorption ntawm DNA mus rau hauv cov kua silica thaum muaj ntsev ntau. Cov khoom siv uas cov kua roj silica ua los ntawm qhov tshwj xeeb, muaj txiaj ntsig zoo thiab tshwj xeeb. Cov khoom siv no yog tsim los rau kev ua kom huv txog 24 μg plasmid DNA los ntawm 1-4 ml hmo ntuj kab lis kev cai ntawmE.coli. Plasmid DNA ua kom huv los ntawm cov khoom siv no tuaj yeem siv rau ntau yam kev sim ib txwm muaj, xws li zom zaub mov zom zaub mov, PCR, ua ntu zus, ligation, hloov pauv, tshuaj xyuas lub tsev qiv ntawv, hauv vitro transcription thiab kev hloov pauv ntawm cov kab mob sib kis ib txwm muaj, thiab lwm yam.

Miv. Tsis yog	Ntim Loj
4992191	50 ua prep
4992192	200 kev npaj ua ntej

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Kev ua haujlwm

Kev sim ua piv txwv

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■ Ceev: Cov txheej txheem yooj yim nrog cov kauj ruam tsawg kawg los ua kom cov plasmid DNA dawb huv tsis pub dhau 8 feeb.
Efficiency Kev ua haujlwm siab: tuaj yeem ntxuav ntau dua 85% plasmid DNA los ntawm E.coli.
■ Cov xim qhia TIANRed tau muab los tswj thiab nco txhua txoj hauv kev thaum sim ua kom ntseeg tau qhov ua tau zoo ntawm plasmid purification.

Txhua yam khoom tuaj yeem tsim kho rau ODM/OEM. Yog xav paub ntxiv,thov nyem Customized Service (ODM/OEM)

Yav dhau los: TGuide S32 Automated Nucleic Acid Extractor

Tom ntej: EndoFree Midi Plasmid Kit

Nrog kev ua haujlwm zoo lysate thiab kev ua kom zoo dua qub, TIANprep Ceev Ceev Mini Plasmid Kit tuaj yeem tau txais cov khoom lag luam zoo dua qub thaum txuag ntau lub sijhawm ua haujlwm.

Kev sib piv ntawm TIANprep Cov Khoom Siv Plasmid Ceev Ceev Mini thiab Cov Khoom Siv A (cov plasmid DNA cov khoom siv ua kom huv) los ntawm kev ntxuav tib plasmid tib lub sijhawm.

Qauv ntim: 3 ml hmo ntuj kab lis kev cai ntawm E.coli (OD600 = 1.8); Kev tshem tawm ntim: 50 μl; Chaw ntim khoom: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

Cov electrophoresis tau ua ntawm 6 V/cm rau 30 min ntawm 1% agarose gel. M: TIANGEN DNA Marker IV.

Xaus: Agarose gel electrophoresis qhia tias TIANprep Ceev Ceev Mini Plasmid Kit tuaj yeem tau txais cov txiaj ntsig zoo ib yam ntawm plasmid DNA raws li los ntawm cov khoom siv plasmid ua kom huv tsis pub dhau 8 feeb.

TIANprep Rapid Mini Plasmid Kit